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Objective To evaluate the correlation between multi-slice spiral CT(MSCT)perfusion imaging parameters and gastric carcinoma differentiation.Methods MSCT perfusion parameters including blood flow(BF),patlak blood volume(PBV),time to peak (TTP)and patlak permeability surface(PPS)were obtained in 44 patients with pathologically confirmed gastric carcinoma before sur-gery.Patients were divided into two groups;26 well differentiated and 18 poorly differentiated.According to the extent of cell differ-entiation ,BF,PBV,TTP and PPS were investigated in two groups.Results Parameters in the well differentiated gastric carcinoma group BF 101.8±6.7 (mL·min-1 ·100 mL-1 ),PBV 85.9±3.4 (mL/1 000 mL ),TTP 121.6±1 1.5(0.1 s),PPS 82.7±1 1.0 (0.5 mL·min-1 ·100 mL-1 )respectivily,Parameters in the poorly differentiated gastric carcinoma group,BF 105.3 ±7.7,PBV 92.1±7.1,TTP 113.0±10.5,PPS 94.3±22.9 respectivily.There was no statistic difference in BF value between the two groups(P >0.05). There were statistic differences in TTP、PBV and PPS between the two groups(P <0.05).Conclusion MSCT perfusion parameters may reflect the degree of differentiation of gastric carcinoma or a certain extent in vivo.
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Objective To evaluate the clinical value of MRA on the abnormal vessels in Moyamoya and cerebral revascularization , and to evaluate PWI in the observation of cerebral hemodynamics before and after cerebral revascularization.Methods Twenty-four patients with Moyamoya disease ascertained by DSA received cerebral revascularization on one side.MRA and PWI were performed for all patients before and after the operation , while DSA was performed after operation in nine patients to compare the images of MRA and DSA by three experienced radiologists.Perfusion parameters in terminal branches of middle cerebral artery ( MCA) on the operative side were compared with those on the contralateral sides and the cerebellum , including cerebral blood flow (CBF), cerebral blood volume (CBV), mean transit time (MTT), and delay time ( DT) , as well as relative ratio ( values on the operative side/values on the contralateral side , and values on the operative side/values on the cerebellar region ) of perfusion parameters (rCBF, rCBV, rMTT and rDT) were calculated.Comparisons of the data between different groups were performed using paired Student′s t test.Results MRA was similar to DSA in displaying the internal carotid artery , main branches stenosis, Moyamoya vessels, and cerebral revascularization.DSA was better in displaying collateral vessels than MRA, but primary images of MRA provided anatomic and pathologic information of cerebral parenchyma.After the operations , rCBF (1.30 ±0.27) and rCBV (1.26 ±0.21) of MCA perfusion regions on the operative and the contralateral sides were higher than rCBF (0.73 ±0.15) and rCBV (0.98 ±0.12) before the operation significantly (t=-7.19,-6.64,P<0.05).rMTT (1.06 ±0.20) and rDT (1.07 ± 0.18) after the operation were lower than rMTT(1.53 ±0.34)and rDT (1.40 ±0.26) before the operation (t=5.62,5.40,P<0.05) .In MCA perfusion regions on the operative and cerebellar sides , rCBF(1.93 ± 0.34)and rCBV(2.25 ±0.35)were higher than rCBF(0.88 ±0.18)and rCBV(1.16 ±0.22)(t=-3.04,-3.06,P<0.05) before the operation.rMTT (1.13 ±0.29) and rDT (1.29 ±0.12) were lower than rMTT (1.88 ±0.19 ) and rDT ( 3.29 ±0.47 ) before the operation ( t =4.01, 4.72, P <0.05 ).Conclusions MRA is a safe , reliable method to evaluate abnormal vessels and cerebral revascularization after the operation in moyamoya disease.PWI can detect poor cerebral perfusion , and can be used for assessment of the effect of cerebral revascularization.
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[Objective] To explore the best method for sequelae neuralgia of herpes zoster.[Method] Randomly divide the said patients into groups treated with external application of Chinese drugs and sky moxibustion,make observation and comparison between them.[Result] Both got high late curative effect,however most patients in the sky moxibustion group were cured without recurrence,the pain relieving rate was high once being cured,the reduce of pain index was apparent after treatment,which was of statistical meaning compared with drug application group.[Conclusion] The sky moxibustion method combines drugs and moxibustion well and has marked advantages in treating herpes zoster,worth of promotion and application.
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Objective: FF456 is a new gene cloned in our lab, which belongs to c-type G protein-coupled receptors and has high homology to the olfactory receptor family. Northern blotting and RT-PCR showed that the expression of FF456 was exclusively restricted in liver and was significantly down-regulated in hepatoma. In an effort to study the function of FF456 gene, high titer antibody is indispensable. Methods:Full-length cDNA of FF456 was reconstructed into pcDNA3.1(-) vector, which was used for immunizing 6- to 8- weeks old female BALB/c mice. The effects of different injection manners, solvents and dosages on the antibody production have been compared. For detecting the titer and specificity of the antisera produced by DNA immunization, a peptide containing FF456 specific sequence was expressed by E.coli expression system. Results:Finally specific antisera with titers as high as 1 ∶ 50 000 was obtained. Conclusion:Immunofluorescence assays showed FF456 was expressed in hepatocytes with high tissue specificity. The FF456 expression in hepatoma cells was decreased dramatically.